本實驗采用雙抗體夾心ELISA法。抗HIV-1 Gag p24單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗HIV-1 Gag p24抗體，標本、標準品中的HIV-1 Gag p24會與加入于孔中的生物素化的抗HIV-1 Gag p24抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有HIV-1 Gag p24，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm 處測OD值，HIV-1 Gag p24 濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中HIV-1 Gag p24度。

產品性能

1. 靈敏度：最小可測人HIV-1 Gag p24達10pg/ml 。

2. 重復性：板內、板間變異系數均<10%。

產品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、微量振蕩器、坐標紙。

• HUMAN ADAMTS1 ELISA KIT（EH052-96）
• HUMAN IL-17R ELISA KIT（EH051-96）
• HUMAN MMP-2 ELISA KIT（EH048-96）

• ELISA kit 產品手冊2019點擊下載

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